Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Expression of integrin αv, β3 in different ovarian tissues.

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Immunohistochemical staining in ovarian malignant tumor ( A1 – A3 ), borderline tumor ( B1 – B3 ), benign tumor ( C1 – C3 ) and normal ovarian tissue ( D1 – D3 ). Integrin αv ( A1 , B1 , C1 , D1 ); β3 ( A2 , B2 , C2 , D2 ) and Lewis y ( A3 , B3 , C3 , D3 ). (Original magnification ×200).

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Immunohistochemical staining, Staining

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Relationship between integrin αv with the clinical and pathological parameters of malignant ovarian cancer.

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Diffusion-based Assay

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Relationship between integrin β3 with the clinical and pathological parameters of malignant ovarian cancer.

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Diffusion-based Assay

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Correlation between expression of Lewis y antigen and integrin αv in ovarian cancer.

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Correlation between expression of Lewis y antigen and integrin β3 in ovarian cancer.

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Integrin αv, β3 and Lewis y colocalize in ovarian malignant tumor. Using an immunofluorescent double-labeling method. Integrin αv and β3 ( A1 and B1 ); Lewis y ( A2 and B2 ); nucleus ( A3 and B3 ); Merged image ( A4 and B4 ). (Original magnification ×400).

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Labeling

Journal: Journal of Experimental & Clinical Assisted Reproduction

Article Title: Molecular mechanisms in uterine epithelium during trophoblast binding: the role of small GTPase RhoA in human uterine Ishikawa cells

doi: 10.1186/1743-1050-2-4

Figure Lengend Snippet: Localisation of E-cadherin and integrin subunit α v . A-D : E-cadherin; E-H : integrin subunit α v , both detected by confocal laser scanning microscopy. Note that both adhesion molecules are located in all plasma membrane domains, including the apical one. Typical patterns are presented for apical (apical) and basal (basal) cell poles as well as for the middle part (mid) of cells. D,H: negative controls.

Article Snippet: Mouse monoclonal antibody against α v integrin subunit (0770) was obtained from Dianova (Hamburg, Germany).

Techniques: Confocal Laser Scanning Microscopy

Journal:

Article Title: Integrin-associated Lyn Kinase Promotes Cell Survival by Suppressing Acid Sphingomyelinase Activity *

doi: 10.1074/jbc.M803301200

Figure Lengend Snippet: Knockdown of the integrin αv subunit reduced OL survival on fibronectin. A, OLs were transfected with αv integrin-specific siRNA or scrambled siRNA (SCR) and cultured for 48 h on fibronectin. αv integrin protein expression was confirmed by Western blotting. Membranes were probed with anti-β-actin antibody to confirm equal loading of samples. B, quantification of the αv integrin expression in transfected cells. Data are means ± S.E. (from four independent experiments). *, p < 0.05 compared with SCR. C, OLs were transfected with αv integrin-specific siRNA or scrambled siRNA and cultured for 48 h in fibronectin (FN)- or laminin (LN)-coated plates. Cell death was measured using an LDH assay. Data are means ± S.E. (n = 18). *, p < 0.05 compared with SCR. D, cells were allowed to attach on fibronectin for 4 h, and then cells were treated withαvβ3 integrin inhibitor and cyclic RGD-containing peptide (cRGD) and cultured for 44 h. Cell survival was measured by LDH assay. Data are means ± S.E. (n = 18). *, p < 0.05 compared with control.

Article Snippet: The OLs were transfected with 25 or 50 n m siRNA targeting the α v subunit of the integrin receptor or scrambled siRNA in an Amaxa Nucleofector device.

Techniques: Transfection, Cell Culture, Expressing, Western Blot, Lactate Dehydrogenase Assay

Journal:

Article Title: Integrin-associated Lyn Kinase Promotes Cell Survival by Suppressing Acid Sphingomyelinase Activity *

doi: 10.1074/jbc.M803301200

Figure Lengend Snippet: OL death induced by αv integrin knockdown is caspase-dependent. OLs were transfected with 50 nm αv integrin-specific or scrambled siRNA (SCR) and cultured in fibronectin-coated plates for 4 h, and then cells were treated with 50 μm caspase-8 inhibitor (Ac-LETD-H), 50 μm caspase-9 inhibitor (Ac-LEHD-H), or 50 μm pancaspase inhibitor (benzyloxycarbonyl-VAD-fluoromethyl ketone) and cultured for 44 h. Cell death was measured using an LDH assay. Data are means ± S.E. (n = 24). *, p < 0.05 compared with SCR.

Article Snippet: The OLs were transfected with 25 or 50 n m siRNA targeting the α v subunit of the integrin receptor or scrambled siRNA in an Amaxa Nucleofector device.

Techniques: Transfection, Cell Culture, Lactate Dehydrogenase Assay

Journal:

Article Title: Integrin-associated Lyn Kinase Promotes Cell Survival by Suppressing Acid Sphingomyelinase Activity *

doi: 10.1074/jbc.M803301200

Figure Lengend Snippet: Knockdown of αv integrin or Lyn kinase induced sphingomyelin hydrolysis and accumulation of C16-ceramide. A, OLs were transfected with 50 nm αv integrin-specific or scrambled (SCR) siRNA and cultured for 48 h on fibronectin. Sphingolipids were analyzed by tandem MS. B, OLs were transfected with 50 nm Lyn-specific or scrambled siRNA and cultured for 48 h on fibronectin with or without 1 μm myriocin (Myr) or 20 μm FB1. Sphingolipids were analyzed by tandem MS. Data are means ± S.E. (n = 18). *, p < 0.05 compared with SCR.

Article Snippet: The OLs were transfected with 25 or 50 n m siRNA targeting the α v subunit of the integrin receptor or scrambled siRNA in an Amaxa Nucleofector device.

Techniques: Transfection, Cell Culture

Journal:

Article Title: Integrin-associated Lyn Kinase Promotes Cell Survival by Suppressing Acid Sphingomyelinase Activity *

doi: 10.1074/jbc.M803301200

Figure Lengend Snippet: Knockdown of theαv integrin or Lyn kinase stimulated ASMase activity, and knockdown of ASMase protected cells from Lyn kinase-dependent cell death. OLs were transfected with 50 nm αv integrin-specific siRNA (A) or with 50 nm Lyn-specific siRNA (B) or scrambled siRNA (SCR) and cultured for 48 h on fibronectin. Then cells were harvested, and ASMase activity was measured. Data are means ± S.E. (n = 9). *, p < 0.05 compared with SCR. C and D, OLs were transfected with SCR or 50 nm ASMase-specific siRNA and cultured for 48 h on fibronectin. Then cells were harvested, and total RNA was isolated and analyzed by RT-PCR. C, OLs were exposed to 20 μm Lyn inhibitor SU6656, and cell death was quantified 24 h later. D, data are means ± S.E. *, p < 0.05 (n = 16). E, OLs were exposed to 20 μm Lyn inhibitor SU6656 with or without 20 μm FB1 or 1 μm myriocin, and cell death was quantified 24 h later. Data are means ± S.E. *, p < 0.05 (n = 12).

Article Snippet: The OLs were transfected with 25 or 50 n m siRNA targeting the α v subunit of the integrin receptor or scrambled siRNA in an Amaxa Nucleofector device.

Techniques: Activity Assay, Transfection, Cell Culture, Isolation, Reverse Transcription Polymerase Chain Reaction